Ecology, Environment and Conservation Paper

Vol. 21 Suppl.Issue August 2015; Page No.(377-380)

A MODIFIED METHOD FOR DNA ISOLATION FROM MADHUCA INSIGNIS (RADLK.) H.J. LAM - A CRITICALLY ENDANGERED SPECIES

K. Souravi and P.E. Rajasekharan

Abstract

Madhuca insignis is a riparian species that is classified as “Extinct”, and was rediscovered after a gap of 120 years. In order to undertake effective conservation strategies, it is important to understand the molecular fundamentals of the species such as the genetic diversity and gene flow within the available wild populations. DNA based analysis is an essential component in any molecular studies such as plant diversity, genetic transformation and clonal fidelity determination of micropropogated plants, methods that have long found their places in plant conservation employing biotechnological tools, Hence this study was undertaken to achieve a good quality and quantity of genomic DNA, to undertake any future studies in this critically endangered species. The quantity of the genomic DNA isolated was found to be high for the small amount of the tissue sample taken. The quality of the DNA was measured by A260/ A280 ratio and also the DNA purity was further confirmed by restriction end nuclease digestion and PCR amplification using operon arbitrary primers (OPC 6, OPC 7, OPC 8 and OPC 11). The results show that the DNA isolated using this method is of good quality and quantity and is amenable to restriction digestion and PCR amplification.

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