Ecology, Environment and Conservation Paper

Vol. 21 Suppl.Issue August 2015; Page No.(251-257)

GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLE USING MOMORDICA CHARANTIA AND MANILKARA ZAPOTA SEEDS

Munmun Karmakar, Kshitiz Kumar, Vijay S. Sharanagat and Ashish Dixit

Abstract

Green synthesis of nanoparticle is a novel way to synthesis nanoparticles by using biological sources. In the present study plant seeds of Momordica charantia (bitter guard) and Manilkara zapota (sapodilla) were taken to investigate their potential for synthesizing silver nanoparticles. The seed extract was mixed with silver nitrate solution of different concentration (10-1 mM, 10-2 mM and 10-3 mM). The silver nanoparticles synthesized were at standard concentration i.e., 10-3 mM and confirmed by their change of colour to dark brown due to the phenomenon of surface plasmon resonance and the characterization of silver nanoparticle was done by UV-Vis spectroscopy. The peak was observed at near to 420 nm and 425 nm for sapodilla and bitter guard respectively. The antibacterial activity of synthesized silver nanoparticles were determined and found that silver nanoparticles have significant antibacterial activity against Bacillus subtilis, Bacillus licheniformis and Bacillus fusiformis. The developed silver nanoparticle from bitter guard seeds showed greater zone of inhibition against Bacillus licheniformis and Bacillus fusiformis which was 14.5 mm and 10 mm respectively. Similarly, silver nanoparticle synthesized from sapodilla seeds showed activity of 14.5 mm against Bacillus subtilis.

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