MOLECULAR CHARACTERIZATION OF ONION (ALLIUM CEPA L.) USING ISSR MARKERSB.S.K. Nikhil, A.S. Jadhav and N.S. Kute
A polymerase chain reaction (PCR) based approach, namely inter simple sequence repeat (ISSR) analysis was applied to 22 genotypes of onion (Allium cepa L.) in order to assess the degree of polymorphism within the genes and to investigate if this approach was suitable for genetic studies of onion. For this study, twenty-two onion genotypes were evaluated for variability using a set of 15 ISSR primers. The polymorphisms in PCR amplification products were subjected to the unweighed pair group method for arithmetic averages (UPGMA). The dendogram constructed from the similarity data grouped the genotypes into different clusters. Considering all the amplified primers a total of 81 scorable amplification products were obtained out of which 69 were polymorphic bands and 12 bands were monomorphic. In this study, it was found that maximum diversity was observed between the genotypes Sel 10 and CSKO 1175. Whereas, maximum similarity was observed between the genotypes Phule Samarth and Sel 24.
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