Ecology, Environment and Conservation Paper

Vol 21, Issue 2, 2015; Page No.(1025-1033)

ISOLATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM RHIZOSPHERIC SOIL AND ITS PHYSIOLOGICAL STUDIES

Lochan Singh and Sanjay Jha

Abstract

Seven phosphate solubilizing bacteria, belonging to Enterobacteriaceae family, capable of utilizing Xylose and rock phosphate as the sole carbon and phosphate source were isolated by enrichment culture method at 28°C in aerobic conditions. This PSB exhibited mineral phosphate solubilizing (MPS) phenotype on xylose and glucose, which are substrates for enzyme glucose dehydrogenase (GDH), as carbon sources. The Phosphate solubilizing capacity of isolates ranged from 385μM-547μM at pH 5.0 in 60-168 hours in Xylose supplemented Tris minimal rock phosphate medium.

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