EFFECT OF LETROZOLE LOADED PLGA NANOPARTICLES ON SEX REVERSAL OF CORTES SWORDTAIL XIPHOPHORUS CORTEZI (CYPRINODONTIFORMES, POECILIIDAE)Harshavardhan D. Joshi, Milind B. Katare, J.K. Pathan, Tanveer Hussain, M.P. Bhendarkar and Karan Kumar RamtekeAbstract The aim of present work was to develop a new Letrozole (LTZ)-loaded PLGA nanoparticles for effective delivery of the drug letrozole for musculinization of fish, as an alternative to commercially available masculinization agent. The LTZ-loaded PLGA NPs was prepared by solvent displacement technique. The particle size of LTZ-loaded PLGA NPs were found to be in the range of 72 nm to 520 nm with average size of 221.5±63.3 nm by using LICOMP particle size analyzer and zeta potential is about 21.35 mV using same same equipment with loading efficiency 43.63±4.63 % using spectrophotometry. In the present study, a series of experiments were carried out to induce masculinization using LTZ-loaded PLGA nanoparticles during the sex differentiation period. Swordtail Xiphophorus cortezi fry were treated with LTZ-loaded PLGA nanoparticles at dosages 5, 25, 50 and 100 mg/Kg diet for 10, 15 and 30 days. The results showed an increase in the proportion of males with dosage and duration of treatment.
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