Ecology, Environment and Conservation Paper

Vol 21, Issue 2, 2015; Page No.(773-776)

STUDY OF GENETIC DIVERSITY IN CHICKPEA (CICER ARIETINUM L.) USING MICROSATELLITE MARKERS

M. Sudharani, S. Nagalakshmi, M. Madhavi and N.V. Naidu

Abstract

Chick pea is one of the most important pulse crops in the world. The aim of present study was to quantify the genetic diversity among five released varieties collected from ICRISAT using microsatellite (SSR) markers. A total of 30 markers utilized in the present investigation. The study revealed that a set of 5 microsatellite (SSR) markers could able to separate the chickpea varieties into 3 clusters. A high level of polymorphism was found with the SSR markers and the polymorphic information content (PIC) ranged between 0.91 to 1.14. The number of alleles amplified were ranged from 2 to 3 with an average of 2.46 per primer. The cultivar ICCV- 37 showed the highest similarity of 55% with cultivar JG-11. The lowest similarity (7%) was found between ICCV-37 and ICCV-10. This analysis is useful in the selection of more diverse parental lines for chickpea improvement programme.

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