Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 2, 2015; Page No.(469-478)

STERILIZATION OF PINEAPPLE EXPLANT FROM SIPAHUTAR, NORTH SUMATERA, INDONESIA (ANANAS COMOSUS L.) AND IN VITRO GROWTH INDUCTION

HARAHAP F., POERWANTO R., SOBIR2, HASRUDDIN1, SURIANI C. , SIALLAGAN J. AND ROHYANA

Abstract

Sipahutar pineapple was derived from Sipahutar, North Tapanuli, North Sumatra, Indonesia, currently endangered. The aims of the research were 1) to obtain optimum sterilization techniques for pineapple explants (Ananas comosus L.) from Sipahutar, 2) growth induction of in vitro shoots with Benzyl Amino Purine (BAP) and Indole Acetate Acid (IAA) plant growth regulators (PGR). This research consists of experiment series, sterilization, growth induction, and in vitro propagation. Sterilization techniques used were of 9 kinds, cleaning the explants, used 5% detergent, and then cleaned with water, continued using different sterilants for different durations. Between sterilan I and II, the explants were washed with sterile distilled water. Explants were cultured on MS medium+ 5 mg L-1 Kinetin + 0.5 mgL-1 IAA for induction of early growth. Growth induction continued with four concentration of Benzyl Amino Purine (BAP) (0, 2, 4, 6) mg L-1 and three concentration of IAA (0, 0.5, 1) mg L-1 were used as a treatment in Murashige and Skoog (MS) medium. The results showed: 1) Treatment by soaking the explants with a 0.008% mankozeb, 0.002% streptomycin sulphate for 1 hour followed by 1.05% NaClO, then peel the outside of explants and put on filter paper to dry, and then planted, produced high aseptic culture (55.6%) and showed the highest growth (22.2%), 2). Giving of BAP and IAA significantly affect to appearance of shoots, number of shoots, number of leaves except shoots height. The best treatment was a combination of MS + 2 mg L-1 BAP + 0 mg L-1 IAA.

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