Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 2, 2015; Page No.(437-442)

RESCUING ENDANGERED POPULATION OF RAMIN (GONYSTYLUS BANCANUS (MIQ) KURZ) VIA ORGANOGENESIS SONDANG HUTAJULU1, SUHARYANTO

SUHARYANTO, RENY HAYATI ZUL, YUHELMADILA YUHELMADILA, MURDANI MURDANI AND ASRI INTSIANA PUTRI

Abstract

Ramin (Gonystylus bancanus (Miq) Kurz. is one of the most valuable wood trees species. Its over exploitation reduces native diversity. Remaining populations in Sumatra and Kalimantan were ex-situ conserved under collaborative project between Sinarmas Forestry/Asia Pulp and Paper and Center for Forest Biotechnology and Tree Improvement, Yogyakarta. The objective of this study is to develop the protocol for organogenesis of ramin. Different explant sources and hormone combinations were tested for callus induction and organogenesis shoot initiation. Induction rate was attained for petiole, stem and leaf (23.8%, 11.1%, and 5.5%, respectively). Callus induction was achieved by employing 2,4-D (3.0 and 6.0 mg L-1) supplemented with coconut water (50 mg L-1) on Murashige and Skoog medium. Callus differentiation occurred on media supplemented with different concentrations of 4-CPPU (0.05-0.25 mg L-1), marked by development of small globular callus. Shoot development was successfully initiated on the combination of 1.0 mg L-1 6-BA and 0.2 mg L-1 NAA. These developed protocols support the propagation for conservation program of this threatened species.

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