Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 2, 2015; Page No.(297-304)

RAMIN (GONYSTYLUS BANCANUS MIQ. KURZ) MICRO PROPAGATION: THE ENDANGERED TROPICAL TREE*

ASRI INSIANA PUTRI M.P.

Abstract

Ramin (Gonystylus bancanus Miq. Kurtz) natural population has been decreasing sharply due to over exploitation, and nowadays has been leading to extinction. The lack of propagation technique information is among the reasons why there has not been enough initiation to develop ramin plantation yet. This research was conducted to ramin micro propagation study by Center of Forest Biotechnology and Tree Improvement (CFBTI), Forestry Research and Development Agency, Ministry of Forest in cooperation to Asia Pulp and Paper (APP). The aim of this study was the effect of growth media on the shoot initiation and growth of ramin in vitro. The ramin explants were from auxiliary shoot seedling. Research design of this study was used Complete Randomized Designed with 5 treatments and 50 replications. Five compositions of growth media used Murashige-Skoog media with benzyl-aminopurine (BAP), 6-furfurylaminopurine (kinetin/K) and napthalene-acetic-acid (NAA). BAP 3 mgL-1 : K 0,15 mgL-1 : NAA 0,5 mgL-1 (H1); BAP 3 mgL-1 : K 0,15 mgL-1 (H2); BAP 3 mgL-1 : NAA 0,5 mgL-1 (H3); K 0,15 mgL-1 : NAA 0,5 mgL-1 (H4) and control (H5). The result showed that medium H1 (1.79 + 0.0141) that has BAP, NAA and Kinetin combination was significantly highest in proliferation coefficient. During 6 months time periods, the percentage of axenic cultures was not significantly different for all medium treatments and it was very low. H1 (47.48+0.0683) was also the highest in percentage initiated explants but not significantly different with H2. H1 (15.56 +0.2830) were not significantly different with H3 (17.67+0.1694) and H4 (14.27+0.2210) for percentage roots initiated, these all medium contain NAA.

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