Ecology, Environment and Conservation Paper

Vol 21, Issue 1, 2015; Page No.(315-319)

ROOT INDUCTION IN POMEGRANATE EXPLANT USING AGROBACTERIUM RHIZOGENES STRAIN RPB13

Nisha M. Patil and S. G. Borkar

Abstract

Induction of rooting in pomegranate air layer graft has always been a problem and therefore preparation of tissue culture explants is being explored for rapid multiplication. However use of costly hormones, less number of roots and survive are main handles in pomegranate tissue culture explant. Use of A. rhizogenes as rooting induction was therefore explored. In vitro A. rhizogenes strain RPB13 treatment at the base of micropropogated explants and improve the rooting of pomegranate (hard wood) plant. A. rhizogenes strain RPB13 demonstrated the highest survival percentage in explant (100%) along with the highest no. of roots (max.18 per explant), whereas in hormonal treated micro propagated explants, the survival percentage was 88% and the no. of roots was max 10 per explant. The induction of rooting in explant by strain RPB13 required less days (18-20 days) than that of hormonal treatment (30-35 days). Also, the no. of roots after six weeks in A. rhizogenes treated explants was 133% more over the hormonal treatment. Thus, A. rhizogenes strain RPB13 has opened an avenue for rooting in micropropagated explants in pomegranate without the hormonal use.

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