DOXYCYCLINE INDUCED ALTERATIONS IN THE GLYCOGEN AND PROTEIN CONTENT OF THE ZEBRA FISH, DANIO RERIO PADMAVATHI VUTUKURU1, ARUN KUMAR BODAPATIVAMSEAKRISHNA BHIMAVARAPU AND S.S. VUTUKURUAbstract Current aquaculture practices potentially lead to increased levels of antibiotic residues and antibiotic-resistant bacteria both in aquaculture products and their immediate environment. Research is warranted on the environmental and health impact of the use of antibiotics in aquaculture. In this study, the toxic impact of doxycycline on the glycogen and protein content of D. rerio exposed to 5, 10, 20 and 40 mg/ L for 24 and 96 h was investigated. Loss of scales, hyper secretion of mucus, loss of balance, irregular swimming patterns were noticed in the fish exposed to 10 mg/L, 20 mg/L and 40 mg/L. Significant decline in the glycogen was observed in the gill and muscle tissues in 20 and 40 mg/L at 24 h while this decrease was observed even at 10 mg/L at 96 h. However, the decrease in glycogen of viscera exposed to all concentrations was insignificant at 24 and 96 h. After 24 h, the total protein content in gill decreased by 17.53%, 30.89%, 48.36% and 66.56%, and by 25.50%, 38.14%, 53.99% and 75.75% from control at 96 h, in 5, 10, 20 and 40 mg/L, respectively. Similar trends were also observed in muscle and viscera. Insignificant decrease was observed in protein content in gill, muscle and viscera of fish exposed to 5 mg/L at 24 h and 96 h. Zebra fish can be effectively used as model for understanding drug toxicity. The drug induced significant changes in glycogen and protein content of gills, muscle, viscera of the fish demonstrating a time concentration relationship. The occurrence of doxycycline at >10 mg/L in aquatic ecosystems is a matter of great concern in public health perspective via transmittance of antibiotic resistant genes.
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