Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(255-259)

MONITORING AND ANALYSIS OF PETROL BIODEGRADATION USING CHROMATOGRAPHIC METHODS

A.H. JOBANPUTRA AND V. R. KOTHARI

Abstract

Due to wide spread use of petrol in situ biodegradation of pollutants has attracted immense attention in the few decades. Oil contaminated soil isolates, identified as Pseudomonas aeruginosa and Acinetobacter lwoffii by 16S ribosomal DNA (rDNA) sequencing and phylogentic anaylsis were used for the present investigation. The bacterium grew optimally at 370C and pH 6.5 and maximum growth was obtained at 5% (v/v) petrol concentration. The intermediate of biodegradation catechol was detected by HPLC. Biodegradation of petrol was monitored by quantitative gas chromatographic analysis. The percent degradation of petrol by Pseudomonas aeruginosa and Acinetobacter lowffi within 25 days was found to be 95% and 97% respectively. These strains were thereby suggestive for application in petrol biodegradations under all investigated nutritional and environmental conditions in polluted soil.

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