Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(227-234)

EEFICIENCY OF TWO SPORE COUNTING METHODS AND VALIDATION OF SSR MARKERS ASSOCIATED WITH SPOT BLOTCH RESISTANCE

G.S. JASUDASU, RAMESH CHAND, A.K. SINGH, V.K. MISHRA AND A.K. JOSHI

Abstract

An experiment was conducted to determine the efficiency of two spores counting methods from wheat seeds. Seeds 40 genotypes collected from the Wheat Association mapping population from the Varanasi for the recovery of Bipolaris sorokiniana spores. The correlation (r) between these two methods was (0.8). Results indicated that Nematode Plate count Method (NPM) was superior to the Washing Test method (WT). The mean recovery of spore in the nematode count plate was significantly higher than the washing test method. In Nematode Plate Method, the spore count distributed with a mean of 126.47/50 seed while in the Washing Test, the spore count mean was 64.15. Recovery of different of black and mixed population was recorded from the seed. White population could not be detected from samples. In the present study, validation and utility of two SSR markers associated with spot blotch resistance in wheat (Triticum aestivum L.) was undertaken. Two primers were used for assessment of genetic variability among 40 wheat genotypes. The phenotypic data in the form of spore load of individual genotypes was compared with the genotypic data. The selected SSR primers showed a high level of polymorphism. Xgwm148 produced 170 bp band in resistant cultivar, while Xgwm111 amplified 150 bp band in resistant cultivar. SSR marker Xgwm148 linked with QTL, QSb.bhu-2B and Xgwm111 is linked with QSb.bhu-7D.

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