Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(215-225)

OPTIMIZATION OF PROCESS PARAMETERS AND MEDIA COMPONENTS TO INCREASE THE BIOMASS OF CYANOBACTERIA (BLUE-GREEN ALGAE) ANABAENA AMBIGUA USING RESPONSE SURFACE METHODOLOGY

M.B. VENKATA RAMANA REDDY, S.S. LAKSHMANA RAO AND C.S. RAO

Abstract

The Biomass of cyanobacteria (blue-green algae) Anabaena ambigua has wide applications as biofertilizers, in immunodiagnostics, and in wastewater treatment. In this study the process parameters and media components of BG-11 media which play a key role in biomass formation are optimized by Response Surface Methodology with Central Composite Design. The variables studied in detail during the growth of cyanobacteria (blue-green algae) Anabaena ambigua were pH, light intensity, sodium nitrate, and calcium chloride. The optimum conditions are pH=7.43, light intensity=80 μmol/m2s, sodium nitrate=1.50g/L, calcium chloride=0.04g/L with a desirability of 0.97. The biomass formed under these conditions is 0.71g/ L The model is validated with coefficient of determination R2=0.9440.

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