Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(209-214)

CHARACTERIZATION AND TOXICITY STUDIES OF BACILLUS THURINGIENSIS AGAINST WHITE GRUB HOLOTRICHIA SERRATA

NAYIMABANU TAREDAHALLI AND A.R.V. KUMAR

Abstract

White grubs (Coleoptera: Scarabaeidae) are a complex set of species responsible for heavy crop damage in many types of cropping systems. These pests being soil borne are extremely difficult to manage even with the best of the insecticides. In the present investigation an attempt was made to isolate some local strains of Bacillus thuringiensis Berliner active against white grubs. A total of 200 Bacillus isolates were obtained from soil samples collected in different parts of Karnataka. The microbiological characteristics of the isolates indicated that isolates were rod shaped endospore formers. Insecticidal effects of the isolates were tested against first instar Holotrichia serrata, provided 18 isolates with 100% mortality of grubs. This investigation has identified 18 local bacterial isolate that can be used as effective biological control agent for the management of white grub pests in the areca nut ecosystem.

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