Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(205-207)

DETECTION OF INDUSTRIALLY IMPORTANT THERMOSTABLE ENZYME OF THERMOPHILES ISOLATED FROM HOT WATER SPRINGS AT VAJRESHWARI, THANE DISTRICT

M.R. PIMPLISKAR, R.N. JADHAV, H.S. VANMALI AND SNEHA MENON

Abstract

Thermophilic microorganisms are of special interest as a source of novel thermostable enzymes. Thermostability is a characteristic of most of the enzymes for industrial usage. Total 12 bacterial strains were isolated from the samples collected from three different sites of hot water springs namely Ganeshpuri Kund, Surya Kund and Ram Lakshaman Sita Kund. Isolates were tested for extracellular enzyme production including gelatinase, urease, amylase, cellulase and catalase. As a follow up studies with these 12 isolates were carried out to detect out their enzymatic activity or enzymatic productivity using standard procedures. From the observations these isolates were found to be novel source of highly active exoenzymes with significant activities at high temperature. Thus these 12 isolates may be regarded as promising source of Industrially important enzymes.

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