Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(139-144)

CATHECOL AND EUGENOL REVERSING THE RESISTANCE OF MRSA TOWARDS THE β-LACTAM ANTIBIOTICS

DEVJANI BANERJEE, TEJAS GOHIL, RACHNA PATEL AND KARISHMA SHAH

Abstract

The present experiment was undertaken to study the effect of bioactive molecules (Cathecol and Eugenol) isolated from Camellia sinensis (Green Tea) and Piper betle (Paan) leaf extracts respectively, on the activity of β-lactamase enzyme produced by Methicillin resistant Staphylococcus aurues (MRSA). Bacterial isolate was obtained from the clinical samples, Anand, Gujarat, India. Extracts of fresh leaves of Camellia sinensis and Piper betle were prepared according to the previous published references. Various biomolecules were separated and identified using TLC and HPLC. Antimicrobial activity and β lactamase activity was checked against the crude extracts and the isolated molecule. Catechol, a derivative of Catechin present in Camellia sinensis was found to have the maximum inhibition on the β-lactamase activity in MRSA. Whereas, Eugenol was docked with penicillin binding protein 2A (PBP2A), which is mainly responsible for the resistant pattern in MRSA species.

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