ISOLATION AND CHARACTERIZATION OF BACTERIA FROM INDUSTRIAL EFFLUENTS AND THEIR TOLERANCE TO HEAVY METALSA.S. KABIR, M.P.E. SHAEKH, F. PARVIN, K.M.F. HAQUE, K.K. NIPA, A.E. EKRAM, M.S. UDDIN AND M.A. SALEH
The pollution of the environment with toxic heavy metals is spreading all over the world along with industrial progress. The use of microorganisms to remove heavy metals from industrial effluents is an area of extensive research; moreover, removal of heavy metals from contaminated sites using microorganisms is a cheaper alternative to chemical technologies. The present study was undertaken for isolation, identification and characterization of heavy metals (Cd2+ and Pb2+) tolerant bacteria from industrial effluents of BSCIC Industrial Estate, Sopura, Rajshahi, Bangladesh. Initially a total of 25 bacterial isolates were screened and only two isolates were selected. Identification of their 16S rRNA sequences showed tohave 96% and 95% similarities to Staphylococcus sp. and Bacillus sp. respectively. These bacteria could tolerate cadmium up to 0.7 mM and 0.6 mM and lead up to 0.6 mM and 0.8 mM correspondingly. They showed their maxium growth at pH 6 and 8 and at 30 oC and 35 oC temperature. Antibiotic sensitivity of these bacterial species was determined against 10 different antibiotics (ampicillin, tetracycline, chloramphenicol, erythromycin, gentamicin, penicillin, vancomycin, ciprofloxacin, amoxycillin, streptomycin.). The Bacillus sp. appeared to be most susceptible being inhibited by eight antibiotics and resistant to penicillin G and amoxicillin on the contrary Staphylococcus sp. was resistant to penicillin G and showed susceptibility to the rest of the antibiotics. These types of bacteria may be useful for the bioremediation of heavy metal contaminated industrial effluents.
Enter your contact information below to receive full paper.