A MOLECULAR SURVEY IN RICE GERMPLASMS FOR MULTIPLE ABIOTIC STRESS TOLERANCE TO CHANGING CLIMATE USING MICROSATELITE (SSR) MARKERSS. Bharathkumar, Jitendra, K., Pragnya, P.J., Sai Krishna R., Chaitanya, K.G. and J.N. Reddy
In PCR screening, gene-specific as well as polymorphic alleles were documented in 122 rice germplasms using SSR markers linked to qSUB1 (submergence tolerance), qSaltol (Salinity tolerance), qDTY2.1 and qDTY3.1(drought tolerance), qAG (anaerobic germination) and qHTS (Heat tolerance). Among these, less number of rice germplasms (only 15%) was identified to be possessed gene-specific allele for submergence tolerance compared to heat tolerance (82%). And, the higest PIC value with RM324 marker for drought tolerance (0.67) and the lowest with RM8094 for salinity tolerance (0.26) were obtained in these genotypes. In this study, many rice germ plasms associated with more than one abiotic stress tolerance were identified by detecting PCR allele in their genotypes. This is why; we report that the rice germplasms with multiple tolerances to various abiotic stresses will be useful genetic resources in rice breeding to adapt rice varieties in changing climate.
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