RAPD ANALYSIS OF FLUORESCENT PSEUDOMONAS ISOLATES FROM APPLE AND PEAR RHIZOSPHERESheetal Rana, Ranjna Sharma and Mohinder Kaur
Molecular markers have been found useful in assessing the genetic diversity in a precise manner, which is based on naturally occurring DNA polymorphism. Random Amplified Polymorphic DNA analysis was studied using four arbitary decamer primers to observe genetic homogeneity/ polymorphism among 10 isolates of fluorescent Pseudomonas sp. isolated from Apple and Pear rhizosphere from different locations of Mandi and Chamba districts. Out of 52 bands generated with 4 RAPD primers 13.46% were monomorphic bands while 86.33% were polymorphic bands. The result showed that the isolates of fluorescent Pseudomonas sp. were divided into 2 clusters by RAPD primers. Among all the combinations maximum similarity (90.48%) was found between PN-7-Cha and PN-2-San isolates of Chamba and Mandi districts. However minimum similarity (36.7%) was observed in PN-11-San and PN-7-Cha isolates of Mandi and Chamba districts.
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