Ecology, Environment and Conservation Paper

Supplement Issue, Dec. 2014; Page No.(127-133)

RAPD ANALYSIS OF FLUORESCENT PSEUDOMONAS ISOLATES FROM APPLE AND PEAR RHIZOSPHERE

Sheetal Rana, Ranjna Sharma and Mohinder Kaur

Abstract

Molecular markers have been found useful in assessing the genetic diversity in a precise manner, which is based on naturally occurring DNA polymorphism. Random Amplified Polymorphic DNA analysis was studied using four arbitary decamer primers to observe genetic homogeneity/ polymorphism among 10 isolates of fluorescent Pseudomonas sp. isolated from Apple and Pear rhizosphere from different locations of Mandi and Chamba districts. Out of 52 bands generated with 4 RAPD primers 13.46% were monomorphic bands while 86.33% were polymorphic bands. The result showed that the isolates of fluorescent Pseudomonas sp. were divided into 2 clusters by RAPD primers. Among all the combinations maximum similarity (90.48%) was found between PN-7-Cha and PN-2-San isolates of Chamba and Mandi districts. However minimum similarity (36.7%) was observed in PN-11-San and PN-7-Cha isolates of Mandi and Chamba districts.

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