Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 4, 2014; Page No.(993-998)

ACUTE TOXICITY EFFECTS OF MWCNTS ON HUMAN LUNG CELLS

AKBAR ZIAUDDIN A., S.K. BEEBIB AND N.A. SIDDIQUIA

Abstract

CNTs (Carbon Nano Tube) are bio-persistent and have the potential to induce severe inflammatory and fibrotic reactions. The published in-vitro studies have given a diverging data. Several studies demonstrated that MWCNTs (Multi Walled Carbon Nano Tube) are able to induce cytotoxic effects and Apoptosis in different cell types. Other studies indicated that there is a very low or no toxic effect. The reason for these discrepancies is not evident probably this may be due to different experimental protocols and interferences with test systems. Hence the present study has standardized the experimental protocol and used the same for all the experiments. Cell viability was analyzed with the MTT, XTT, Apoptosis assays. These tests were conducted after the incubation of MWCNT’s in an aqueous solution of fetal bovine serum and in the absence of fetal bovine serum. The results indicated that the cells in serum free media were affected greatly by MWCNTs rather than serum containing media.

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