Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 4, 2014; Page No.(983-987)

AN EFFICIENT PLANT REGENERATION USING SHOOT TIP EXPLANTS OF RAUWOLFIA SERPENTINA (L.) - AN ENDANGERED ANTIHYPERTENSIVE MEDICINAL PLANT

N. CHANDRALEGA, D. SUBHA AND N. GEETHA

Abstract

Rauwolfia serpentina L. (Sarpagandha) is an endangered woody perennial shrub belonging to Apocyanaceae family as a source of antihypertensive drug. Due to the high demand and over harvesting of raw materials of this medicinal plant in pharmaceutical industry, there is an urgent need to develop an efficient protocol to overcome exhaustion of R. serpentina. The shoot tip explants were cultured on MS medium fortified with different concentrations of (0.5-2.5 mg/L) BAP and KIN either solely or in combination with 0.5mg/L NAA. The highest frequency of shoot regeneration (90.56%), multiple shoot bud induction (14.63 shoots/ explant) and shoot length (7.35 cm) were obtained, when these were cultured on MS medium augmented with 1.5 mg/L BAP in combination with 0.5 mg/L NAA. After four weeks, in vitro grown shoots were transferred to MS medium supplemented with various concentrations of NAA (0.5-2.0mg/L) for rooting. MS medium containing 1.5 mg/L NAA showed the best response of rooting (94.63 %), maximum number of roots (8.34 roots/shoot) and root length (8.78 cm) within two weeks. The plantlets were hardened in plastic cups containing sterile soil and sand in the ratio of 2:1. After hardening, the plantlets were successfully transferred to field conditions with 95% survival rate. This protocol were established for in vitro plant regeneration from shoot tip explants of R. serpentina and it may be suitable for high frequency of shoot multiplication of this endangered antihypertensive medicinal plant.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!