Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 4, 2014; Page No.(887-894)

SUSCEPTIBILITY PROFILE OF BACTERIA ISOLATED FROM DIABETIC WOUND TO SILVER NANOPARTICLES SYNTHESIZED USING MURRAYA KOENIGII

S. ANANTHA SUGANIYA AND R. MALA

Abstract

The major problem of wound management and therapy is bacterial infection and wound sepsis, which in worst case may, lead to mortality. Proper management of wound and successful wound healing requires the identity of colonizing bacteria and its sensitivity to antibiotics, as most of the pathogens have developed resistance to commonly used antibiotics. Thus the present work is aimed to evaluate the prevalence, etiology and susceptibility pattern of bacterial pathogens isolated from diabetic wounds to commonly used antibiotics and silver nanoparticles. The sample was collected from Government Hospital in Aruppukkottai, Virudhunagar District, Tamilnadu, India. Silver Nanoarticles were synthesized using Murraya koenigii. Out of 5 pathogenic isolates, predominant pathogen (3) was Pseudomonas aeurogenosa. Others were Enterobacter sp and Staphylococcus sp. Antimicrobial susceptibility test for isolates were executed using disk and well diffusion method. Pathogens were resistant to gentamycine, neomycine, cephalosporin, penicillin, metrogel, silvergel and ciproflaxacin. But sensitivity was intermediate to ciproflaxacin and susceptible Silver Nano particles. Nano particles were characterized by XRD and FTIR. MIC of Silver nano particles (SNP) to the isolated P. aeruginosa ASM 100 was 1.5mg/mL and MBC was 6mg/mL. So the present study reveals that Pseudomonas was highly resistant to most of the drugs used to treat infection. Susceptibility towards silver nano particle opens a window for new wound dressing with SNP synthesized using edible leaf.

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