Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 4, 2014; Page No.(841-846)

EXPERIMENTAL INVESTIGATION ON ENHANCED PRODUCTION OF PECTINASE BY IMMOBILIZED ASPERGILLUS NIGER USING LOW COST SUBSTRATE

N. KAVITHA, V. SUDARSHANA DEEPA, T. CHITRA DEVI AND R. RAVIKUMAR

Abstract

The present study investigated the production of pectinase by immobilized Aspergillus niger NFCCI 2822 in submerged fermentation using low cost substrates of carrot and corn. Batch experiment was conducted at 30°C with various substrate concentrations at pH 4.0 with different time intervals to obtain the maximum yield of Pectinase. Results revealed that the medium containing carrot yielded maximum pectinase at 72 hours than the other one. Pectinase produced by carrot medium which was partially purified had the maximum enzyme activity of 147.02 U/mL than enzyme produced by the corn medium of 103.68 U/mL.

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