Ecology, Environment and Conservation Paper

Vol 20, Issue 3, 2014; Page No.(1059-1065)

INFLUENCE OF RHIZOBIUM INOCULATION ON N, P AND K CONTENT IN DALBERGIA SISSOO ROXB.

Anish Kumar, D. Dash and M. K. Jhariya

Abstract

The present experiment was conducted in glass house of Department of Microbiology, College of Agriculture, Raipur, Chhattisgarh in the year 2009-10 with six treatments comprised of Dalbergia sissoo-Rhizobium inoculation @ 5 mL inoculum/seedling, 2 levels of N fertilization (N1 and N2 as 2g and 4g N/seedling) alone and along with each other including one control to evaluate the response of Dalbergia sissoo to Rhizobium inoculation and the influence of nitrogen on biological nitrogen fixation. This involved isolation of Rhizobium isolate from nodule of D. sissoo. Results revealed that inoculation with Dalbergia sissoo-Rhizobium and nitrogen fertilizer application influenced the nutrient concentration in different seedling components. Rhizobium inoculation showed higher N concentration over uninoculated ones. Significantly higher N, P and K content in shoot of D. sissoo (216.0, 13.62 and 159.23 mg/seedling, respectively) was found when N1 was applied along with inoculation. Root inoculation of D. sissoo seedlings with species Rhizobium showed significant effect and further application of N at lower level along with Rhizobium inoculation was found most effective in terms of increasing symbiotic traits and nutrient uptake by D. sissoo seedlings.

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