IN VITRO SCREENING OF ANTIBACTERIAL ACTIVITY OF FERRULA ASAFOETIDA EXTRACTSOMER MOHAMED KHALILAbstract Petroleum ether, methanol and water extracts of Ferrula asafoetida were prepared and tested against Staphylococcus aureus (ATCC 25923), Escherichia coli, (ATCC 25922) Pseudomonas aeruginosa (ATCC 25922) refrances strains in addition to 50 clinical isolate . The cup-plate agar diffusion method was adopted. The inhibition zones were compared with that of standard antibiotics amoxicillin and neomycin. The minimum inhibitory concentration (MIC) of aqueous and methanolic extract was determined. Phytochemical screening of the plant was carried out. Petroleum ether expressed no activity against all bacterial strains as compared with other solvents. Methanolic and water extracts revealed a range of 21 to 24 mm and 15 to 20 mm inhibition zone respectively. Concerning the activity against the clinical isolates, methanolic extract showed 76 %, while water extract was only 40% activity. Staphylococcus aureus is the most sensitive microorganism tested, with the lowest MIC value (7.45 mg/ mL) in the presence of the methanolic extract. The Phytochemical screening of methanolic and water extracts contain high concentration of tannin compounds. Therefore, such screening experiments form a primary platform for further phytochemical and pharmacological studies that may open the possibility of finding new clinically effective antibacterial compounds.
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