Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(641-646)

DEGRADATION AND DECOLORIZATION OF MAGENTA DYE BY BACTERIAL SOIL ISOLATES

REKHA GUPTA, ADITI DESHPANDE AND YASHODA YAMGAR

Abstract

Present investigations were undertaken to study biodegradation and decolorization of magenta dye using soil isolates. Soil sample was collected from the site contaminated with magenta dye and bacterial isolates were obtained. The maximum degradation and decolorization of the dye was shown by one of these bacterial isolates (isolate M5) which was later characterised to be Pseudomonas aeruginosa. The decolorizing ability of this isolate was confirmed by carrying out plate assay. Degradation and decolorization of magenta dye was obtained upto 87% in 10 hours (after treatment) under static conditions. The spectrophotometric analysis and thin layer chromatographic studies confirmed the degradation of the dye. Estimation of biochemical oxygen demand (BOD) and chemical oxygen demand (COD) were also carried out as a measure of oxygen required by the micro-organisms to degrade the organic content and at the same time to find out the amount of organic content present. Studies on phytotoxicity of the degradation product on the germination of seeds of Phaseolus mungo proved that the product may be non- toxic.

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