Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(633-639)

A FACILE ONE-POT SYNTHESIS OF GOLD NANOPARTICLES BY CHLAMYDOMONAS REINHARDTII

M. DIVYA RAO AND GAUTAM PENNATHUR

Abstract

We report a simple green method for the biosynthesis of gold nanoparticles by the reduction of aqueous chloroauric acid solution with the extract of Chlamydomonas reinhardtii. The gold nanoparticles were characterized using UV-Visible spectroscopy, High resolution transmission electron microscopy (HR-TEM) and Fourier Transform Infra Red spectroscopy (FT-IR). The gold nanoparticles were found to have an absorption maxima at 540 nm and the HRTEM micrograph indicated the formation of well dispersed spherical particles with diameter ranging from 9-40 nm.The presence of algal proteins as capping and reducing agents was determined using FT-IR spectroscopy. The SDS-PAGE profiles of the intracellular proteins indicated the presence of four intense bands ranging from 26 -32 kDa and these proteins may be responsible for the synthesis and stabilization of the gold nanoparticles

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