Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(627-632)

UTILIZATION OF CASSAVA SAGO WASTE FOR BIOETHANOL PRODUCTION BY CO-FERMENTATION OF STARCH DEGRADING BACTERIA AND SACCHAROMYCES CEREVISIAE

R. PRAVEEN KUMAR, B. BHARATHIRAJA, K. PRAGADEESH, S. CHOZHAVENDHAN AND A. WILLIAM JOHNSON

Abstract

Among various environmental issues, waste management is a leading area where the effective and intensive utilisation of wastes and obtaining useful products in biological ways are concentrated. Biochemical characterization of sago industry waste shows that it is rich in carbohydrates, in more specific it contains 55-60% of starch. This work deals with the management of waste for bioethanol production. Native microorganisms were used for converting the sago starch into simple sugars. Amongst them, six bacterial strains were found effective for starch degradation. The cofermentation to produce ethanol was carried out in six different sets with 5% load of isolated bacterial strains and later by addition of 1% load of Saccharomyces cerevisiae. All the experiments were carried out in laboratory scale Erlenmeyer flasks, incubated in shaking incubator for five days at 35°C. The results show that the strain 3 yielded a biomass of 17.041 g/L thereby producing 2.01 g/L of ethanol.

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