Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(621-626)

ENHANCED BACTERICIDAL PROPERTY OF SILVER NANOPARTICLES SYNTHESIZED FROM PENICILLIUM CITRINUM (MTCC 2547) AND EVALUATE IN COMBINATION WITH CARBICILLIN AND AMOXICILLIN

ANIMA NANDA AND SHAHNAZ MAJEED

Abstract

Nanotechnology is the branch of science and technology which deals with the particles which are in nanosize have contained improved properties due to size distribution and morphology. An important area of nanotechnology is the development of reliable and eco friendly process for synthesis of nanoscale particles through biological systems. In the present work we report the biological synthesis of silver Nanoparticles (AgNPs) from Penicillium citrinum (MTCC 2547) by the extracellular method. The color of the solution changes in to dark brown in the conical flask indicates the formation of Silver Nanoparticles. These Silver Nanoparticles were analyzed by the UV-Vis Spectroscopy which detects the presence of Silver Nanoparticles in the solution. These Silver Nanoparticles were further characterized by Fourier Transform Infrared (FTIR) Spectroscopy, Field emission scanning electron microscopy (FESEM) which shows that the average particle size is in the range of 17 to 20nm. These synthesized silver Nanoparticles have been checked with for its anti bacterial activity against various bacterial pathogens. These synthesized silver nanoparticles have been compared with Carbicillin and Amoxicillin. The synergistic activity of the Carbicillin and Amoxicillin with the synthesized Silver nanoparticles was found to be more effective and increases the antibacterial activity of Carbicillin and Amoxicillin against the selected pathogenic bacteria studied.

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