Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(601-610)

ISOLATION AND CHARACTERIZATION OF A NOVEL L-GLUTAMINASE PRODUCING MARINE BACILLUS SUBTILIS STRAIN JK-79

JAMBULINGAM KIRUTHIKA AND NACHIMUTHU SARASWATHY

Abstract

A novel marine bacterial strain JK-79 capable of producing extracellular L-glutaminase enzyme was isolated from marine soil collected from Parangipettai, East costal region of India by enrichment and spread plate technique sequentially. It was identified as Bacillus subtilis JK-79 (KC492745) based on the morphological, physiological-biochemical characteristics, 16S rDNA sequences and phylogenetic analysis. The L-glutaminase production was carried out by submerged fermentation. Maximum L-glutaminase production (268 U/mL) was recorded at pH 7, 24 hours, 37°C, 2% inoculums concentration and 2.5% glutamine concentration. When different carbon sources were added as supplements, the enzyme production was considerably increased with 2% fructose upto 313U/mL. Different organic and inorganic nitrogen sources were supplemented and the enzyme production was increased with 2% yeast extract (258U/mL).

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