SULPHUR OXIDIZING BACTERIA (SOB): OPTIMIZATION OF THEIR PHYSIOLOGICAL CHARACTERISTICSPERIYASAMY RAMESHKUMAR, SHANMUKHANAND POTHANA, SURULIRAJ MANIKANDAN AND GOVINDASAMY MANIVANNAN
In order to optimize their physiological characteristics of a novel new sulphur oxidizing bacterium acquired from cattle manure compost. The present study assumed to their growth, pH, and temperature, utilization of different sulphur sources, sulphur oxidizing capacity and requirement of oxygen. The results of the present work show that the SOB grows much faster and more rapidly reaches to stationary phase at 24 hours incubation. The optimum growth extends at the time of 24 hours (50% of thiosulphate, 1054 µg/mL proteins content and 8.8×103 numbers of cells were observed). Conductivity (219mV) and acid contents (2.64 mM) were significantly greater in the pH of 3. The optimum temperature for their growth was around 35oC-40oC. Sodium thiosulphate has predominant energy source for SOB. Sulphate ions production (50.0%, 55.5% & 48.1%) and retained thiosulphate concentration (55.9%, 50.2%, and 50%) were observed at pH-3, 30oC for 24 hours respectively. The oxygen-sufficient conditions (OSC) support for their growth than the oxygen-limiting conditions (OLC).
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