Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(497-507)

OPTIMIZATION OF BIOMETABOLITE PRODUCTION BY STREPTOMYCES SP PT1 IN SUBMERGED CULTURE FOR IMPROVED IN VITRO ANTIBACTERIAL AND ANTI-OXIDANT ACTIVITY

ZIDAN ABDULDIEM BASHIR, GIRES USUP, ASMAT AHMAD AND SHUKOR MD NOR

Abstract

A 30-run Box-Behnken design (BBD) was applied to statistically optimize the production of biometabolite from Streptomyces sp PT1 in submerged culture by varying four factors: nitrogen (yeast extract) concentration, MnCl2, FeSO4 and NaCl. Two antimicrobial assays (using Bacillus subtilis and Vibrio parahaemoliticus as test organisms) and three anti-oxidant assays were incorporated to test for the activity of the produced biometabolite. For antimicrobial assay conducted using Bacillus subtilis (BS) as test organism, the optimum extraction conditions consisted of a basic medium composed of: nitrogen (10 g/L), FeSO4 7H2O (0.35 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For CUPRAC and DPPH antioxidant assays, optimum results were found with the use of a basic medium comprising of: nitrogen (20 g/L), FeSO4 7H2O (1.0 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (2.0 g/L). For FRAP antioxidant assay, optimum results were found with the use of a basic medium comprising of: nitrogen (2 g/L), FeSO4 7H2O (0.135 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For the metabolite produced by Streptomyces sp PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was thus found effective to optimize medium components producing up to 66.11%, 34.29%, 12.46%, 28.44% and 27.53% increase in responses for antimicrobial (B. subtilis, Vibro parahaemoliticus) and antioxidant assays (FRAP, CUPRAC and DPPH) respectively.

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