Pollution Research Paper

Vol 33, Issue 02, 2014; Page No.(487-492 )

ANTI - BIOGRAM AND RESISTOGRAM PROFILING OF AEROMONAS SPECIES ISOLATED FROM MALAYSIAN COASTAL SEAWATER

OLUMIDE ADEDOKUN ODEYEMI* AND ASMAT AHMAD

Abstract

Antibiotics and heavy metals resistance profile of Aeromonas species isolated from Kuala Gula and Kuala Sepetang, Perak, was investigated in this study.Isolated Aeromonas species were subjected to 12 antibiotics: Penicillin G 10 units, Tetracycline 30 μg, Novobiocin 5 μg, Nitrofurantoin 100 μg, Ampicillin 10 μg, Chloramphenicol 10 μg, Fluconazole 25 μg, Gentamicin 10 μg, Streptomycin 10 μg and 25 μg, Kanamycin30 μg, and Vancomycin 30 μg and 5 heavy metals: MnSO4.H2O; Cr(NO3).9H2O; CuSO4; Pb (NO3)2; HgCl2.Results from this study revealed that-isolates from the two sources-displayed varied resistance. Isolates from Kuala Sepetang showed 91%, 66%, 61%, and 52 % to Vancomycin, Novobiocin, Streptomycin-and Gentamycin while Kuala Gula isolates showed 76%, 93%, 28% and 14 % respectively.High rate of resistance to lead (Pb), Chromium (Cr), copper (Cu) and Manganese (Mn) with minimum inhibitory concentration ranging from 2560μg/mL to >5120 μg/mL. Low resistance to mercury was observed among isolates from Kuala Gula.This study showed that there was widespread of both heavy metals and antibiotics resistance among Aeromonas isolates in coastal seawater from Kuala Sepetang showing more resistance thereby posing danger to both aquatic animals and human health.

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