Ecology, Environment and Conservation Paper

Vol 20, Issue 2, 2014; Page No.(633-637)

COMPARATIVE STUDY OF FUNGAL DNA EXTRACTION PROTOCOLS IN DIFFERENT FUNGAL STRAINS

S. Janaki Alias Priya1, Anurag Chaturvedi, Manorama Kanuri, Ravicharan Ameda, Prathima Namburi, Sreedhar Mulinti, M.R. Krishna Bhagavatula and Sunil Chidambar Kulkarni

Abstract

Mycotoxins are fungal metabolites, which are chemically diverse and occur in a wide variety of food and fodder. Fungal toxins are low molecular weight chemical compounds which are not detected by antigens, and hence produce no obvious symptoms. Mycotoxins are insidious poisons. Major mycotoxin-producing fungal genera, are Aspergillus, Fusarium, and Penicillium. The present study was undertaken with the main aim of comparing CTAB method and stratagene DNA extraction kit method for extraction of DNA from six different Fungal Strains. Commercial development and use of DNA extraction kits has greatly simplified sample preparations. DNA was extracted from Aspergillus parasiticus, Aspergillus ochraceus, Fusarium culmorum, Fusarium verticillioides, Fusarium graminearum and Fusarium moniliforme using Stratagene DNA extraction kit and CTAB method both were found to be efficient and reproducible and also yielded DNA of high molecular weight with low levels of fragmentation and high quality.

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