Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(405-409)

PRODUCTION AND PURIFICATION OF POLYPHENOLOXIDASE FROM ASH GUARD PEEL

ZOYA JAVED, SHAILENDRA KUMAR SHRIVASTVA AND GYAN DATTA TRIPATHI

Abstract

Polyphenol oxidases are enzymes that catalyze the oxidation of certain phenolic substrates to quinones in the presence of molecular oxygen. Polyphenol oxidases are widely used in several applications like food industry, medicine, wastewater treatment etc. In this study, detection, purification and characterization of polyphenol oxidase from thermophilic Bacillus subtalis, which was isolated from soil samples. Chemical analysis of Ash Gourd had been performed the chemical component, Protein 12.4 mg, Total soluble sugar 1.1mg, Moisture Content 93.1% and Organic content 0.61% was recorded. By using these values a media was standardized for the growth of bacteria which was signfinently used for production of Polyphenol Oxidase enzymes. This enzyme was further purified by DEAE Cellulose followed by Ammonium sulphate precipitation and dialysis.

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