Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(399-403)

COMPARATIVE STUDY OF DNA EXTRACTION METHODS IN MARINE FISH SPECIES

N. PRATIMA, ANURAG CHATURVEDI, K. MANORAMA, M.R. KRISHNA BHAGHAVATHU AND M. SREEDHAR

Abstract

High quality DNA extractions are required for genetic studies. Eight commercially important fish species were chosen for molecular studies. Species and population genetic assessment requires a reliable source of biological material. Different tissues may contain varying amount of DNA (e.g. scales, muscles tissues) which may not be equally dealt with by different extraction methods. A number of commercial kits for DNA extraction are available. Routine protocols have also been developed for many different tissues in different species. Yet it is often necessary to establish which is the most appropriate DNA extraction method for a certain tissue in a given research context. In the present study, DNA extracted from eight marine fish species were carried by a standard method using CTAB, a rapid phenol chloroform method, and an advanced method for fish DNA extraction. Quantity of DNA was higher in the standard methods, but adequate quantity and quality of DNA obtained when DNA was extracted by the advanced fish DNA extraction method.

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