Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(379-380)

A STUDY ON PSEUDOMONAS AERUGINOSA BIOFILM FORMATION USING ATOMIC FORCE MICROSCOPY

ASHWIN KANNAN, SUBRAMANIAN RAMALINGAM AND PENNATHUR GAUTAM

Abstract

Biofilms are bacterial cells in a matrix of extracellular polymeric material. They are formed at airliquid or liquid-solid interface. Formation of biofilms is undesirable since it causes failure of implants and biocorrosion of pipelines. Hence studies on biofilms are important. In this study, the adhesion force of Pseudomonas aeruginosa biofilms was quantified using Atomic Force Microscopy. The adhesion force of 2 days and 4 days old biofilm were 6.9 ± 0.2 nN and 11.8 ± 0.7 nN respectively. The results indicate that the adhesiveness of biofilm increases with incubation time.

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