Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(321-327)

EXPLORATION OF INDUSTRIALLY IMPORTANT ENZYME PRODUCING MICROORGANISMS FROM EXTREME HABITATS OF DIBANG VALLEY, ARUNACHAL PRADESH, INDIA

KODAPARTHI ASHWITHAA, B., G. BALAKRISHNAA, PAVAN KUMAR PINDI A. AND A. SWAROOPA RANIB

Abstract

Microbial diversity constitutes the most extraordinary reservoir of life in the biosphere that we have only just begun to explore and understand. A preliminary investigation on the microbial diversity of potential extracellular enzyme producers was done by direct screening methods to provide an insight on the possibility to use different moderate extremophiles as a source of extremophilic enzymes in biotechnological processes. Samples were collected randomly from soil sediments of Dibang valley, Arunachal Pradesh, India and their physico-chemical characteristics such as pH, moisture content and Nitrogen, Phosphate and Potassium content were determined. The dynamics of microbial population was investigated by using serial dilution method on different microbiological media to ensure maximum microbial diversity. The isolated bacteria, actinomycetes and fungi were screened for their cellulase, protease and lipase activities. The study revealed that bacterial isolates showing higher zone of cellulolytic activity belong to Bacillus, Pseudomonas and Serratia sp, while fungal isolates mostly belong to Aspergillus and Penicillium sp. The study has suggested that the Potential enzyme producing isolates were identified indicating that these extreme habitat soils are a rich source of industrially important enzyme producers.

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