Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(301-306)

ISOLATION AND CHARACTERIZATION OF RHIZOBIAL ISOLATES FROM THE RHIZOSPHERIC SOIL OF AN ENDANGERED PLANT MEIZOTROPIS PELLITA

PANKAJ, TAPAN KUMAR NAILWAL, LALIT SINGH AND AMIT PANWAR

Abstract

Meizotropis pellita (vern., Patwa) an endangered plant species found on the three sites in Patwadanger Nainital Uttarakhand. Meizotropis pellita is an angiospermic plant which belongs to family fabaceae. Most of the fabaceae family plants fix atmospheric nitrogen. Patwa belong to this family, so rhizobacterial spp. from its rhizospheric soil were isolated and characterized. This study focusses on the important role of Rhizobacteria associated with the Rhizospheric soil of Meizotropis pellita. By the biochemical properties of the Rhizobial isolates it is proved that these Rhizobial isolates have potent plant growth promoting abilities (PGPR). PGPR properties of Rhizobial isolates is based on their phosphate solubilisation property. All strain strain showed phosphate solubilisation property. Seven different rhizobacterial strains of bacteria were isolated from the rhizosphere of Patwa plant. Biochemical characterization was done for their identification, and molecular characterization authenticated that the isolated bacteria can fix atmospheric nitrogen and this may have PGPR activity. nif genes expresses and produce nitrogenase enzyme which is mainly responsible for nitrogen fixation. In this study we used PCR amplifications of nif genes present in the isolated bacterial strains. All seven isolate showed nif gene amplification. This is the first study associated with the Rhizospheric region of the M. pellita plant.

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