Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 2, 2014; Page No.(285-293)

ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF DUNALIELLA BARDWIL

A. JENITTA AND S. JAYASURIYA

Abstract

An ideal antioxidant should be readily absorbed and quench free radicals, and chelate redox metals at physiologically relevant levels. The present study is focussed on the antioxidant and free radical scavenging properties of Dunaliella bardawil. The objective of the present study is to evaluate the antioxidant activity of D. bardawil using seven methods viz., DPPH radical quenching method, H2O2 scavenging assay, NO radical scavenging assay, Reducing capacity assay and FTC method, TBA method and Total phenolic content. Ascorbic acid, α-Tocopherol and vitamin C were used as standards. The methanolic extract of D.bardawil showed strong DPPH radical scavenging activity of 86.72±0.33%value with IC50 value of 2.25±0.11 μg/mL and in H2O2 scavenging activity of 94.24±1.33% with IC 50 value of 3.88±0.67 μg/mL, NO radical scavenging activity with 63.55±0.52% with IC 50 value of3.22±0.28 μg/mL. The Dunaliella extract with concentrations higher than 4 mg/mL showed a higher reducing power than alpha-tocopherol and BHA.A volume of 800μL of 2.5% linoleic acid in 99.8% ethanol in a total volume of 20mL, was found to produce the highest absorbance readings and both showing a slightly reduced absorbance after the 8th day. Maximum generation of lipid hydroperoxides was achieved by incubation at 37°C (body temperature). In Green phase the contents of phenolic compounds (mg/g) in aqueous extracts of D. bardawil was found 482 .18±12.30 mg GAE/g dry wt and in red phase525±30.19 mg GAE/g dry wt. Dunaliella showed relatively fair antioxidant potential according to FTC and TBA method but comparatively low DPPH radical scavenging activity. From all these observations it can be concluded that, the algal extracts with high level of polyphenolic compounds and β carotene in Dunaliella show excellent antioxidant activity In vitro systems.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!