ALKALINE PROTEASE PRODUCTION BY NATURAL ISOLATE - THE BACILLUS SP.R. SHARMA AND R. SINGHAbstract Unexplored Purvanchal region of the Uttar Pradesh State was selected as a sampling site just to evaluate the potentialities of the natural isolate of the Bacillus sp. A natural isolates of Bacillus Sp. isolated and identified up to genus level which was found to be a potential producer of alkaline protease enzyme. Three types of media namely synthetic complex and modified were used and finally complex medium was selected through out the entire investigation as it was giving promising result during preliminary examination of the enzyme production by shake flask fermentation method. The carbon, nitrogen and their concentrations play vital role in enzyme production along with cultural conditions like pH, temperature, rpm, volume to container ratio and concentration of active inoculum. With all the optimized parameters the maximum yield was observed about 1450 units/mL with 24 hrs. of fermentation.
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