Ecology, Environment and Conservation Paper

FACTORS AFFECTING CLONAL MULTIPLICATION IN KOKAM (GARCINIA INDICA CHOICY.)

P.B. Vanave, R.L. Kunkerkar and M.B. Khamkar

Abstract

In vitro studies in Kokam (Garcinia indica) were carried to standardize protocol for aseptic culture shoot establishment of Kokam. During the investigation, treatments for surface sterilization, explant size, stage of explants and gelling agents were studied. Studies revealed that the HgCl2 at 0.1% for 4 minutes and HgCl2 at 0.1% concentration for 5 minutes were found to be at par as the best surface sterilization treatments to obtain maximum Kokam shoot establishment in vitro. The shoot tip explant of length 4 cm in length, dark green coloured were the ideal explants for in vitro establishment. Incorporation of Phytagel as a gelling agent in the Murashige and Skoog's medium exerted good conditions for in vitro shoot establishment in Kokam.