Ecology, Environment and Conservation Paper

Vol. 20 Issue 01, 2014; Page No.(361-364)

VALIDATION OF SSR MARKERS LINKED TO LATE LEAF SPOT AND RUST IN GROUNDNUT (ARACHIS HYPOGAEA L.)

Shivaleela Kukanur, D. L. Savithramma, Sheela Duddagi, A. Vijayabharathi, B.P. Mallikarjuna1 and H.D. Pushpa

Abstract

The low productivity of Groundnut in India is ascribed to many biotic and abiotic stresses in the cultivation of the crop. Among the biotic stresses, the two major foliar diseases viz., late leaf spot (Phaeoisariopsispersonata [(Berk. and Curt.) Deighton] and rust (Puccinia arachidis Speg.) causing yield loss up to 50-70 per cent (Subrahmaniyam et al., 1984). The experiment was conducted at UAS, Bengaluru during 2011 and 2012 to validate the markers IPAHM 103, GM 2301 and GM 1009 identified to be linked to rust resistance and late leaf spot resistance respectively in different genetic backgrounds i.e., validating these markers over a set of resistant and susceptible genotypes for their utilization in marker assisted selection (MAS). During kharif 2011 minicore along with other advanced breeding lines were phenotypically screened for late leaf spot and rust disease using modified 9 point scale (Subbarao et al., 1990). Few genotypes consisting of minicore accessions, advanced lines and local cultivars were selected for validation of the above mentioned markers. The DNA extraction was done as per CTAB method given by Saghai-Maroofet al. (1984). Polymerase Chain Reaction (PCR) was performed by using a Touch-Down PCR. Through single marker analysis it was found that these markers IPAHM 103, GM 2301 and GM 1009 were found to be associated with rust resistance and late leaf spot resistance respectively in this study. Hence these markers can be effectively used for MAS for selecting the resistant genotypes inearly generations of resistant breeding.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 27, Issue 1, 2021
Vol 27, Feb Suppl. Issue, 2021
Vol 26, Issue 4, 2020
Vol 26, Nov Suppl. Issue, 2020
Vol 26, Oct Suppl. Issue, 2020
Vol 26, Issue 3, 2020
Vol 26, Aug Suppl. Issue, 2020
Vol 26, Issue 2, 2020
Vol 26, June Suppl. Issue, 2020
Vol 26, April Suppl. Issue, 2020
Vol 26, Issue 1, 2020
Vol 26, Feb Suppl. Issue, 2020
Vol 25, Issue 4 2019
Vol 25, Issue 3 2019
Vol 25, Nov Suppl. Issue, 2019
Vol 25, Sept Suppl. Issue, 2019
Vol 25, Aug Suppl. Issue, 2019
Vol 25, July Suppl. Issue, 2019
Vol 25, Issue 2 2019
Vol 25, May Suppl. Issue, 2019
Vol 25, April Suppl. Issue, 2019
Vol 25, Issue 1 2019
Vol 24, Issue 4 2018
Vol 24, Issue 3 2018
Vol 24, Issue 2 2018
Vol 24, Issue 1 2018
Vol 24, March Suppl. Issue 2018
Vol 24, Feb. Suppl. Issue 2018
Vol 23, Issue 4, 2017
Vol 23, Nov. Suppl. Issue 2017
Vol 23, Sept. Suppl. Issue 2017
Vol 23, Issue 2, 2017
Vol 23, Issue 3, 2017
Vol 23, Issue 1, 2017
Vol 23, Feb 2017 Suppl. Issue
Vol 22, Dec 2016 Suppl. Issue
Vol 22, Issue 4, 2016
Vol 22, Sept. Suppl. Issue , 2016
Vol 22, Issue 3, 2016
Vol. 22, June Suppl. Issue 2016
Vol 22, Issue 2, 2016
Vol. 22, April Suppl. Issue 2016
Vol 22, Issue 1, 2016
Vol 21, Issue 4, 2015
Vol. 21 Dec. 2015 Suppl. Issue
Vol. 21 Nov. 2015 Suppl. Issue
Vol 21, Issue 3, 2015
Vol 21, Issue 2, 2015
Vol. 21 Suppl.Issue August 2015
Vol 21.Suppl.Issue June 2015
Vol 21, Issue 1, 2015
Supplement Issue, Dec. 2014
Special Issue-2014
Vol 20, Issue 4, 2014
Vol 20, Issue 3, 2014
Vol 20, Issue 2, 2014
Vol. 20 Issue 01, 2014
Vol. 19 Issue 04, 2013
Vol. 19 Issue 03, 2013
Vol. 19, Issue 02, 2013
Vol. 19, Issue 01, 2013
Vol.18, Issue 04, 2012
Vol.18, Issue 3, 2012
Vol.18, Issue 2, 2012
Vol.18, Issue 1, 2012
Vol.17, Issue 4, 2011
Vol.17, Issue 3, 2011
Vol.17, Issue 2, 2011
Vol.17, Issue 1, 2011
Vol.16, Issue 4, 2010
Vol.16, Issue 3, 2010
Vol.16, Issue 2, 2010
Vol.16, Issue 1, 2010
Vol.15, Issue 04, 2009
Vol.15, Issue 03, 2009
Vol.15, Issue 02, 2009
Vol.15, Issue 1, 2009
Vol.14, Issue 04, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 1, 2008
Vol.14, Issue 2-3, 2008
Vol.13, Issue 04, 2007
Vol.13, Issue 2, 2007
Vol.13, Issue 1, 2007
Vol.12, Issue 4, 2006
Vol.12, Issue 3, 2006
Vol.12, Issue 2, 2006
Vol.12, Issue 1, 2006
Vol.12, Issue 01, 2006
Vol.11, Issue 3,4, 2005
Vol.11, Issue 2, 2005
Vol.11, Issue 1, 2005
Vol.10, Issue 04, 2004
Vol.10, Issue 03, 2004
Vol.10, Issue 02, 2004
Vol.10, Issue 01, 2004
Vol.09, Issue 04, 2003
Vol.09, Issue 03, 2003
Vol.09, Issue 02, 2003
Vol.08, Issue 04, 2002
Vol.08, Issue 03, 2002
Vol.08, Issue 01, 2002
Vol.07, Issue 04, 2001
Vol.07, Issue 03, 2001
Vol.07, Issue 02, 2001
Vol.07, Issue 01, 2001
Vol.06, Issue 04, 2000
Vol.06, Issue 03, 2000
Vol.06, Issue 02, 2000
Vol.06, Issue 01, 2000
Vol.05, Issue 04, 1999
Vol.05, Issue 03, 1999
Vol.05, Issue 02, 1999
Vol.05, Issue 01, 1999
Vol.04, Issue 1,2, 1998
Vol.03, Issue 3,4, 1997
Vol.03, Issue 01, 1997
Vol.02, Issue 1,2, 1996
Vol.01, Issue 14, 1995
Looking for Past Issues? Click here to get them!!