ATTENUATING EFFECT OF VITAMIN E AND C ON LIVER FUNCTION MARKERS IN SO2 EXPOSED MALE RATSMadhuri Yadav, Asha Agarwal, Meenu Agarwal and Rajni Singh
Present study was carried out to assess the effects of SO2 on liver of albino rats and role of vitamin E and C in combination. Thirty adult wistar albino rats (150-200g) were divided into three groups (1, 2 and 3) of five rats each. Rats of control group (1) were kept in ambient air and rats of experimental group (2) were exposed to 80ppm SO2 gas for 1 hour/day for 4 weeks and rats of experimental group (3) were exposed to 80ppm SO2 gas with supplementation of vitamin E and C in combination orally (0.1ml/100g b.wt. vitamin E and 0.2ml/100g b.wt. vitamin C). After 4 weeks of treatment, the rats were starved overnight and sacrificed by decapitation next morning for studying the liver histopathology and serum enzyme activities. The histopathological changes in rat liver after 4 weeks SO2 exposure include- moderate hepatocyte necrosis (HN), degeneration of hepatocytes (DH), centrilobular necrosis (CN), ballooning degeneration (BD) and cellular debris. Significant elevation in level of serum AST (P<0.05), ALT (P<0.01), and ALP (P<0.05) activity in comparison to control rats. The reduction in hepatotoxicity and serum enzymes activity after oral supplementation of antioxidant vitamin E and C in combination is the result of antioxidant defence mechanism against toxic action of SO2 gas which reduces the intensity of inflammation like responses in SO2 gas induced rat.
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