Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 1, 2014; Page No.(109-113)

RESPONSE SURFACE METHODOLOGY FOR OPTIMIZATION OF BIOSURFACTANT PRODUCTION FROM BACILLUS SP. VITPGMB ISOLATED FROM MARINE WATER

MEENAKSHI SINGH GRUSHA, PIYUSH JAIN, L. KARTHIK, G. KUMAR AND K.V. BHASKARA RAO

Abstract

The aim of the present study was to isolate microorganism that produces biosurfactant and to optimize its production. Lipase and hemolytic activity were performed as primary screening procedures. Three potential isolates (GMP1 to GMP3) were subjected to secondary screening by Drop collapsing method, Oil displacement method and Emulsification index. A decrease in surface tension at oil-water interface was recorded. Microbial Enhanced Oil Recovery (MEOR) was performed and oil elution recorded from GMP1 and GMP2 was 2.0 mL and 2.2 mL respectively. GC-MS analysis of the extract from GMP2 revealed the presence of surface active compounds. Response Surface Methodology (RSM) was used to study the interactions between various factors and it was found that a pH=8.91, temperature = 25.66°C, peptone - 4.04 g/L, yeast extract - 3.09 g/L and concentration of oil - 0.52% were optimal for production from GMP2. The isolate was identified as Bacillus sp. VITPGMB.

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