Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 1, 2014; Page No.(41-46)

A COMPARATIVE STUDY ON THE PROTEASE PRODUCING BACTERIA ISOLATED FROM DAIRY EFFLUENTS OF CHENNAI REGION, IDENTIFICATION, CHARACTERIZATION AND APPLICATION OF ENZYME IN DETERGENT FORMULATION

MASI CHANDRAN, M. FAZIL AHMED AND N. PARTHASARATHI

Abstract

Protease is an economically important enzyme that is being used in many food industries, pharmaceuticals and manufacturing industries. Since protease has heavy demand commercially, its production reaps riches. Studies were made in different strains producing protease enzyme. We have made an attempt to extract protease from the bacterial strains isolated from dairy effluents of Chennai region. Totally 6 dominant strains were isolated from dairy effluents collected from Aavin milk factory of Pattaravakam, Madhavaram and Sholinganallur of Chennai. Since, milk and milk products contain major amount of casein, bacteria isolated from the dairy effluents are expected to produce more protease enzyme. Bacillus subtilis (MTCC No.1790) was used as the control. We checked and compared the protease activities of isolated strains with the control. We found that Pseudomonas species were more potent in protease production than the other isolated bacterial strains and the control.

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