DETECTION AND IDENTIFICATION OF GIBBERELLA ZEAE TRICHOTHECENE BIOSYNTHESIS GENE TRI13, FOR MEASURING DON PRODUCTION IN INFECTED MAIZE SAMPLES USING NORMAL PCRS. Nagalakshmi, K. Manorama, Naveen Kumar and P. Sravanti, CH V. Durgarani and V.L.N. Reddy, M. Sreedhar, Sunil Kulkarni, Anurag Chaturvedi and A. RavicharanAbstract Maize (Zea maysL.) most important cereals in the world Trichothecenes are sesquiterpenoid compounds produced by fungi from fusarium species are of significance with respect to food safety because many are important pathogens and colonizers of plants, particularly cereal. Trichothecenes include deoxynivalenol (DON) (also known as vomitoxin). The trichothecenes are Gibberella zeae F. graminearum, F culmorum are producers of the type B trichothecenes that are most often associated with infected plant products.The present study was undertaken with the main aim of detecting the presence of the genes encoding the production of mycotoxin, namely, vomitoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Gibberella zeae F. graminearum, F culmorum were collected from the markets, godowns and farmersÂ’ fields. DNA isolated from such maize samples was amplified in PCR using four sets of forward and reverse gene specific primers designed using DNASTAR Lasergene 8.0 version software from original gene sequences (obtained from GENBANK) of the specific gene, Tri13. A 168 base pair fragment of Tri13 target sequence was amplified in thirteen samples in agarose gel electrophoresis. This study helps in easy detection of mycotoxins present in the contaminated samples in storage These assays include conventional PCR, in the near future the molecular technologies will be useful as a preventive approach to minimize the entry of vomixin into the food chain.
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