Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

STATISTICAL SCREENING OF MEDIUM COMPONENTS BY PLACKETT-BURMAN EXPERIMENTAL DESIGN FOR BIOSURFACTANT PRODUCTION BY INDONESIAN INDIGENOUS BACILLUS SPDSW17

SWASTIKA PRAHARYAWAN, DWI SUSILANINGSIH AND KHASWAR SYAMSU

Abstract

Statistical screening of media components for biosurfactant production by Indonesian indigenous of Bacillus spDSW17 was carried out using Plackett-Burman experimental design. Nine out of eleven factors of the production medium were found to be significantly affecting the production process of biosurfactant. FeSO 4.7H 2O, NaNO3 , frying oil waste, CaCl 2.2H 2O, K2HPO4 and sucrose were directly proportional to the biosurfactant production while ZnSO 4.7H 2O, KH2PO 4 and MgSO 4.7H 2 showed inversely proportional correlation with the biosurfactant production in the selected experimental range. Several modified mediums were formulated based on the statistical screening result in order to validate the optimum media composition in producing biosurfactant. Modified medium combination 1 (MM1) as an optimized medium (2.13 g/L) showed 124% increase in biosurfactant production over the unoptimized medium or MM4 (0.95 g/L).