Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 4, 2013; Page No.( 789-793)

MOLECULAR DETECTION OF OCHRATOXIN PRODUCING FUNGAL GENE, POLYKETIDE SYNTHASE GENE(PKS) IN INFECTED MAIZE SAMPLES USING PCR

NAGALAKSHMI S, MANORAMA K , ANURAG CHATHURVEDI , SREEDHAR M , KRISHNA BHAGAVATULA M R C, SUNIL-CHIDAMBAR K , RAVICHARAN A , PRATHIMA N , DURGARANI CH. VaAND REDDY VLN

Abstract

Corn production was estimated a production of around 790 million tonnes. Most of the corn is used for feed, food, and seed,maize (Zea maysL.) is one of the cereals which serves as a main source of food, forage and processed products for industry. As a staple food it provides more than one-third of the calories and proteins in some countries. Ochratoxins are cyclic polyketides, the most potent of which is ochratoxin A (OTA). OTA has been associated with human endemic neuropathy .Large number of other Penicillium and Aspergillus species have since been reported to be OTA producers. The present study was undertaken with the main aim of detecting the presence of the genes encoding the production of mycotoxin, namely, ochratoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus andPenicillium were collected from the markets, godowns and farmersÂ’ fields. DNA isolated from such maize samples was amplified in PCR using sets of forward and reverse gene specific primers designed using using DNASTAR Lasergene 8.0 version software from original gene sequences (obtained from GENBANK) of the specific gene,pks.fragments. A 200 base pair fragments were amplified in eleven samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!