MOLECULAR DETECTION OF OCHRATOXIN PRODUCING FUNGAL GENE, POLYKETIDE SYNTHASE GENE(PKS) IN INFECTED MAIZE SAMPLES USING PCRNAGALAKSHMI S, MANORAMA K , ANURAG CHATHURVEDI , SREEDHAR M , KRISHNA BHAGAVATULA M R C, SUNIL-CHIDAMBAR K , RAVICHARAN A , PRATHIMA N , DURGARANI CH. VaAND REDDY VLN
Corn production was estimated a production of around 790 million tonnes. Most of the corn is used for feed, food, and seed,maize (Zea maysL.) is one of the cereals which serves as a main source of food, forage and processed products for industry. As a staple food it provides more than one-third of the calories and proteins in some countries. Ochratoxins are cyclic polyketides, the most potent of which is ochratoxin A (OTA). OTA has been associated with human endemic neuropathy .Large number of other Penicillium and Aspergillus species have since been reported to be OTA producers. The present study was undertaken with the main aim of detecting the presence of the genes encoding the production of mycotoxin, namely, ochratoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus andPenicillium were collected from the markets, godowns and farmers fields. DNA isolated from such maize samples was amplified in PCR using sets of forward and reverse gene specific primers designed using using DNASTAR Lasergene 8.0 version software from original gene sequences (obtained from GENBANK) of the specific gene,pks.fragments. A 200 base pair fragments were amplified in eleven samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.
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